《植物生理学报》 2010, 46(3): 263-267

利用Imaging-PAM研究莱茵衣藻对环境变化的响应

段晓琼¹, 唐秋菊¹, 褚胜利², 杜林方^1,*

1 四川大学生命科学学院, 生物资源与生物环境教育部重点实验室, 成都610065; 2 上海泽泉科技有限公司, 上海200333

通信作者：杜林方；E-mail: dulinfang@yahoo.com；Tel: 028-85415 008

摘　要：

本文介绍了 Imaging-PAM-M-Series 调制叶绿素荧光成像技术在莱茵衣藻活体叶绿素荧光检测中的应用。该方法利 用CCD 对藻体直接成像, 可同时检测多个样品。后期分析可获得图像中任意区域的初始荧光产量(Fo)、充分暗适应后PSII 的最大光化学效率(Fv/Fm)、PSII光化学能量转化的有效量子产量[Y(II)]、光化学淬灭系数(qP)、非光化学淬灭系数(NPQ)等 指标。文章以敌草隆[3-(3,4-dichlorophenyl)-1,1-dimethylurea, DCMU]和没食子酸丙酯(propyl gallate, PG)对莱茵衣藻野生 型及其抗 DCMU 突变体的影响为例, 说明该技术在莱茵衣藻研究中是可靠的, 具有简单、快速、灵敏等特点。

关键词：Imaging-PAM; 莱茵衣藻; 叶绿素荧光

收稿：2010-01-15   修定：2010-02-01

资助：教育部新世纪人才资助计划(NCET-04-0861)和四川大学“985 ” 计划项目。

Research of Physiological Responses of Chlamydomonas reinhardtii to Environmental Changes Utilizing Imaging-PAM

DUAN Xiao-Qiong¹, TANG Qiu-Ju¹, CHU Sheng-Li², DU Lin-Fang^1,*

¹Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, School of Life Sciences, Sichuan University, Chengdu 610065, China; ²Zeal Quest Scientific Technology Co., Ltd., Shanghai 200333, China

Corresponding author: DU Lin-Fang; E-mail: dulinfang@yahoo.com; Tel: 028-85415 008

Abstract:

The application of Imaging-PAM in the study of physiological responses of Chlamydomonas reinhardtii to environmental stresses was introduced. This technique can image several samples immediately and simultaneously. With this technique the minimum fluorescence yield (Fo), the maximal efficiency of PSII in a dark-adapted state (Fv/Fm), the effective quantum yield of PSII [Y(II)], the photochemical quenching parameter(q_P) and the nonphotochemical quenching parameter (NPQ) can be monitored. A case with the effect of DCMU[3-(3,4-dichlorophenyl)-1,1-dimethylurea] and PG (propyl gallate) on Chlamydomonas reinhardii wide-type strain and its DCMU-resistant mutant was carried. It demonstrated that this technique was reliable, simple, rapid and sensitive.

Key words: Imaging-PAM; Chlamydomonas reinhardtii; chlorophyll fluorescence